This article looks at the treatment of virginity and desire in John Lyly’s late 16th century play Gallathea, a mythological story in which two young women both cross-dress as boys to escape being a virginal sacrifice, and thus fall in love with each other. In this play, Venus (as the proponent of erotic desire) more specifically through the agency of Cupid urges all characters, including Diana’s nymphs, toward romantic love, while Diana (in theory supporting the position of virtue) valorizes virginity and chastity.